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Rheology & microfluidics of lung fluids: the cases of mucus and of pulmonary surfactant

Rheology & microfluidics of lung fluids: the cases of mucus and of pulmonary surfactant

By In PhD proposals 2019 On March 29, 2019


Project: Rheology & microfluidics of lung fluids: the cases of mucus and of pulmonary surfactant

Laboratory: Laboratoire Matière et Systèmes Complexes

Affiliation
Affiliation: Université Paris-Diderot (Paris 7)
Address: UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet, 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13
Website: http://www.msc.univ-paris-diderot.fr/
E-mail: laurent.limat@univ-paris-diderot.fr

LAB Director
Name: Laurent Limat
Phone number: 0157277000
E-mail: laurent.limat@univ-paris-diderot.fr

Supervisor
Name: Jean-François Berret
Phone number: 0157276147
E-mail: jean-francois.berret@univ-paris-diderot.fr

Subject Keywords: Respiratory distress syndrome – Lung fluids – Microfluidics – Microrheology – Human mucus
Summary of lab’s interests: The research at MSC laboratory covers many fields of soft condensed matter including biological physics, complex fluids and dynamics of out-of-equilibrium systems. Located in the Physics Department at University Paris-Diderot, MSC counts 80 permanent researchers, 40 PhDs and postdocs. In biophysics, activities are on cell biomechanics, tissue engineering and nanomaterial development for medicine and biology. In terms of equipment, MSC disposes of modern tools in microscopy (AFM, confocal, optical, fluorescence, electronic), spectroscopy (scattering), and cell culture (L2+).
Project summary: The goal of this project is to study the mechanical and rheological properties of lung lining fluids under healthy and pathological conditions. These fluids are the mucus in the conductive zone and as the pulmonary surfactant in the respiratory zone. They are secreted by the lung epithelium and are the first barrier against pathogens and particles. The results will allow predicting the fluid transport processes in lungs and will help in determining new diagnosis and therapeutic procedures, in particular in case of acute respiratory distress syndrome. The project aims to study the rheological properties of mucus and surfactant lung fluids using advanced microfluidic and microrheology techniques. Lung fluids altered chemically by the presence of inhaled particles or biologically by addition of drugs will be investigated. Human pulmonary mucus samples are collected from the bronchial tubes of patients who have had a lung transplantation or lobectomy surgery (Bichat hospital).
Interdisciplinary aspect of the project: The goal of this project is to use a μ-rheology technique to investigate scarce fluids, here pulmonary fluids whose mechanical properties are directly related with their functions. The project is at the crossroads of soft condensed matter physics, microfluidics, biochemistry and medicine. It aggregates the Berret’s group at MSC and the Norel’s group at the Laboratory for Vascular Translational Science, INSERM, both affiliated at the Université Paris Diderot. Nanotechnology studies will be included through the long-term collaboration with Pr. Armelle Baeza-Squiban from the Biology Department. Inherently, our proposed investigations are interdisciplinary requiring expertise in physics, chemistry, biology and engineering.
Funding: Funding has been requested through he Angence Nationale de la Recherche (ANR)