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Principles of fluorescence techniques

Description

Course Outline:
  • The fluorescence mechanism
  • Fluorophores and fluorescent proteins: which to chose for which application; phototoxicity, improving SNR
  • The fluorescence as a quantitative tool, advanced fluorescence techniques:
    • FLIM
    • Colocalization
    • FRET
    • FRAP
    • Photoactivation Processes and related techniques PALM, STORM, RESOLFT
    • Anisotropy
Schedule that could be shaped depending on the students interest.
First day:
  • Morning
Intro on Fluorescence and fluorophores
Colocalization, FRET and FLIM
  • Afternoon
Software and ImageJ plugin to analyse colocalization images, FLIM images, FRET images
Second day
  • Morning:
FRAP
Photoactivation
Anisotropy
  • Afternoon
Software and ImageJ plugin to analyse FRAP, Photoactivation, Anisotropy

Coordinators

Valentina Caorsi

E-mail: valentina.caorsi@gmail.com

Organization

Dates: 19-20 April 2018 (2-full days)
Location: Room 21.K (21st floor), Montparnasse
Credits: 14 hours
Validation: Students must attend all two days to validate the hours. 

Registration

Enroll on Google Classroom

  • Log in with you cri-paris.org email address
  • Click the + in the top right corner of the screen and click Join class
  • Enter registration code: v6o5td