Pancreatic beta cell purification through innovative microfluidic approaches
Internship title: Pancreatic beta cell purification through innovative microfluidic approaches
Name: Control of pancreas development Cochin Institute and Laboratory Colloïdes et Matériaux Divisés ESPCI
Affiliation: INSERM and ESPCI
Address: Cochin Institute 124 Bd Port Royal Paris and ESPCI, 10 Rue Vauquelin, Paris
Name: Raphael Scharfmann and Jérôme Bibette
Phone number: 33176535568
Name: Raphael Scharfmann and Klaus Eyer
Phone number: 33176535568
Subject Keywords: Beta Cell, Insulin, Purification, secretome, microfluidic
Tools and methodologies: Pancreatic islet preparation;
Insulin-producing beta cell purification through the development of microfluidic approaches
Transcriptomic and physiological analyses of the purified cells
Summary of lab’s interests: The Scharfmann’s lab at Cochin is interested the regulation of functional beta cell mass (the pancreatic cells that produce ans secrete insulin) that are destroyed in patient with type 1 diabetes. Klaus Eyer and colleagues at ESPCI have recently developed innovative approaches to purify cells based on their specific secretome.
Project summary: Insulin producing beta cells (β-cells) are endocrine cells located in the pancreas. They play a major role in physiology by secreting insulin, the sole hormone that decreases glycemia in mammals. Due to their complex pancreatic location and the lack of specific cell surface markers, β-cells are extremely difficult to purify to homogeneity, which limits their study at the cellular level.
Recently, scientists at ESPCI, Paris, developed innovative approaches to purify cells based on their specific secretome (doi: 10.1038/nbt.3964). This represents a breakthrough when compared to previous approaches and opens new avenues to purify cells on hormones they secrete.
Within a collaborative effort between INSERM (Scharfmann’s team, Cochin Institute (https://www.institutcochin.fr/departments/emd/team-scharfmann?set_language=en) and ESPCI (Jérôme Bibette, Laboratory ‘Colloïdes et Matériaux Divisés’, https://www.lcmd.espci.fr), we aim at developing an approach to purify β-cells based on their unique property to secrete insulin.
Our preliminary data demonstrate the feasibility of the approach. Such a new approach will be useful for many purposes. As a first example, it will permit to compare at the transcriptional and functional levels neonatal (immature) and adult (mature) β-cells, an approach to pave the way for generating functional beta cells from stem cells.
Interdisciplinary aspect of the project: The Cochin and ESPCI teams have different, but synergistic expertises. The team at Cochin is expert is pancreatic beta cell biology. The team at ESPCI has expertise in cell purification through innovative microfluidic approaches. During the past 12 months, we collaborated with the objective of using the expertise of ESPCI to discover innovative ways to purify beta cells. Our first data obtained within this interdisciplinary collaboration support the feasibility of this innovative approach.