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High throughput single cell characterisation

High throughput single cell characterisation

By In Aiv Internship On July 22, 2019


Internship title: High throughput single cell characterisation

LABORATORY
Name: Gulliver
Affiliation: ESPCI
Address: 10 rue Vauquelin
E-mail: yannick.rondelez@espci.fr

LAB Director
Name: Olivier Dauchot
Phone number: 0140795105
E-mail: olivier.dauchot@espci.fr

SUPERVISOR
Name: yannick Rondelez
Phone number: 0140795105
E-mail: yannick.rondelez@espci.fr

Subject Keywords: Single cells, Microfluidics, molecular programming, Next generation sequencing, molecular biology
Tools and methodologies: Single cells, Microfluidics, molecular programming, Next generation sequencing, molecular biology
Summary of lab’s interests: This project is a collaboration between two groups, the group of Andrew Griffiths, with a strong expertise in single-cell high-throughput approaches, and the group of Yannick Rondelez, developing new methods to build complex molecular systems with applications, for example, in diagnostics or molecular evolution.
Project summary: High throughput single cell methods open new windows on a variety of biological domains, including immunology, transcriptomics, bacteriology, etc. Molecular barcoding strategies are an essential component of these approaches: unique DNA tags are attached to individual objects at the beginning of the protocol and retrieved by sequencing at the end. This allows the biochemical steps to be applied at millions of individual molecules in parallel, while keeping the possibility to re-assign each of them to its original sub-population. However, creating and attaching these barcodes constitute in many cases a bottleneck, a cost and a source of noise. The goal of this internship will be to explore an innovative barcoding strategy that can address these pitfalls.
Interdisciplinary aspect of the project: This project draws on many modern techniques in molecular science. It combines the expertise of two groups and will allow the intern to explore many lab techniques, in particular microfluidics, next generation sequencing, molecular programming and possibly single cell characterization in the context of immunology.