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DNA detection for all

DNA detection for all

By In Aiv Internship On June 28, 2018


Internship title: DNA detection for all

LABORATORY
Name: U1001
Affiliation: INSERM Descartes CRI
Address: 24 rue de faubourg st jaques/ 10 Charles V
E-mail: research@cri-paris.org

LAB Director
Name: Ariel lindner
Phone number: 0617296126
E-mail: ariel.lindner@cri-paris.org

SUPERVISOR
Name: Guy Aidelberg
Phone number: +33769620863
E-mail: guy.aidelberg@cri-paris.org

Subject Keywords: Isothermal amplification
Democratization
Citizen Science
Education
GMO’s/Endangered/invasive species plant/animal pathogens
Tools and methodologies: Isothermal amplification
Open Hardware
Fluorescence
Nucleic Acid detection
Learning by doing/seeing/discussing
Summary of lab’s interests: The CRI is creating a research Collaboratory at the crossroads of life, learning, and digital sciences.

We are developing an open, collaborative research program to tackle the world’s health and education challenges, focusing on the following broad topics, amenable to bridge foundational research and societal impact:

Open synthetic and systems biology – from foundational understanding of living systems to open biotech and open pharma solutions.
Open AI – Understanding and shaping current digital transition in context of learning, health and/or human-machine paradigms.
Open health – from data-rich research to development of frugal software and hardware solutions.
Open learning – from understanding learning to human-machine paradigms
Open phronesis – tackling ethical challenges of our time.
Project summary: The DNA base pair is currently joining the atom, the bit and the joule in the pantheon of elementary units from which new things are made. But DNA is not like the others. Everything we eat, everyone we love, sickness and health, the entire living world is written in DNA. Few today understand, but many fear, this new power that is quickly maturing. To this end, we are developed an easy to use, economic and robust method to allow anyone anywhere to detect a DNA sequence of interest. We have created a 5 minute DNA extraction protocol that only needs water. This is coupled to novel isothermal DNA amplification techniques, detected through fluorescence, utilizing ultra affordable LEDs and plastic gel filters. The first use case is detection of GMO’s in food. This is being expanded to invasive and endangered species as well as plant/livestock pathogens for subsistence farmers. The quick experiment allows anybody to actually see with their eyes if a gene is present. Be it in a school, biohackerspace, a kitchen or the feild, users are encouraged to upload, share and discuss your results with others across the world. Empowering and educating citizens, while allowing new information to be gathered and shared on a global scale.
Interdisciplinary aspect of the project: This project involves many disciplines including novel molecular biology techniques, Open hardware and Data, Citizen Science, new ways of learning and doing research. Interns could test sensitivity and selectivity of tests, develop new assays for new targets, find new interesting targets, give and develop workshops for high school students and the general public, develop and improve new open hardware, develop and improve data analysis and sharing platform etc… Depending on the specific students interests skill and motivations.