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Bacterial cell cycle checkpoints under stress conditions

Bacterial cell cycle checkpoints under stress conditions

By In Aiv Internship On June 19, 2018


Internship title: 

Bacterial cell cycle checkpoints under stress conditions

LABORATORY
Name: CIRB, équipe Chromosome dynamics
Affiliation: Collège de France
Address: 11 place Marcelin Berthelot, 75004 Paris
E-mail: sylvie.rimsky@college-de-france.fr

LAB Director
Name: Olivier Espéli
Phone number: 0144271249
E-mail: olivier.espeli@college-de-france.fr

SUPERVISOR
Name: Sylvie Rimsky
Phone number: 0144271317
E-mail: sylvie.rimsky@college-de-france.fr

Subject Keywords: Bacterial cell cycle
stress respons
persistance
pathogenic bacteria

Tools and methodologies: microscopy
genomic
bacterial culture
microfluidic
Summary of lab’s interests: Our laboratory is interested in the analysis of the cell cycle and chromosome dynamics in response to stimuli or stresses coming from the cytoplasm or the environment. We use a combination of genetic, genomic and cell biology tools and microscopy to study cell cycle, chromosome conformation and persistance to antibiotics.
Project summary: We observed that survival of Entero-pathogenic bacteria inside macrophages is strongly dependent on two general stress pathways: the SOS response and the stringent response (SR). These pathways influence the number of surviving cells but also the number of non-growing (persisters) bacteria among the population. Both SOS and Stringent response have been extensively studied, however, independently from each other. Since little is known about their interconnections. For example there is no data to tell if the two responses are occurring simultaneously at the single cell level or in the population.
We propose to monitor the interplay between SR and SOS with genetic and genomic tools combined with cell imaging and fluorescent reporters. During her/his master the student will measure the induction of the two systems and analyse the impact on the cell cycle, the number of persister cell and the viability in pathogenic and commensal bacteria.

Interdisciplinary aspect of the project: This project combine a range of different approaches from classical microbiology, fluorescent microscopy including super resolution, microfluidics to high throughput genomic studies.