A 3D microfluidic tumour model to study colon cancer heterogeneity and drug resistance
Internship title: A 3D microfluidic tumour model to study colon cancer heterogeneity and drug resistance
Name: Translational Research and Microfluidic Team, UMRS1138, Centre de Recherche des Cordeliers
Affiliation: Paris Descartes university
Address: 45 rue des Saints Pères 75270 Paris Cedex 06
Name: Valerie Taly
Phone number: 0170649954
Name: Valerie Taly, Wenjin Xiao
Phone number: 0170649973
Subject Keywords: Microfluidics, Spheroids, Tumour-on-a-chip, Cancer heterogeneity, Drug resistance
Tools and methodologies: Microfluidics, Cell Imaging, Flow cytometry, Cell biology, Molecular biology
Summary of lab’s interests: The translational research and microfluidic team (TRAM) group, directed by Dr. Valérie Taly, conducts research based on the miniaturization of biological and chemical reactions for cancer research with the aim of applying the developed tools and procedures in clinics. This highly interdisciplinary team is composed of scientists with biology, chemistry or physics backgrounds and works in very strong interaction with oncologists and clinicians.
Project summary: Multicellular tumour spheroids (MCTSs) have been highlighted as pertinent in vitro cancer models especially for analysing drug efficacy. Recently, microfluidics have demonstrated the potential to bridge the existing in vitro-in vivo gap by providing the platforms allowing spatiotemporal manipulation of the cellular microenvironment in order to precisely control tumour tissue formation. The objective of this internship is dedicated to studying colon cancer intra-tumour heterogeneity and its role in drug resistance using a microfluidic 3D tumour model. The aims of the proposal is to: 1. develop and optimise a microfluidic platform to generate multicellular tumour spheroids (MCTSs) with physiological relevant heterogeneity by co-culturing cancer cells with different levels of drug sensitivity; 2. characterise the multicellular tumour spheroids and investigate the cell-cell crosstalk in promoting MCTSs evolution and drug resistance using multilevel sensing techniques, including confocal microscopy imaging of e.g., cell viability assays, immunoassays and flow cytometry; 3. investigate the particular role of extracellular vesicles in the tumour progression and drug resistance.
Interdisciplinary aspect of the project: This project combines microfluidics, bioengineering, synthetic biology, cell biology, molecular biology and oncology to develop cutting-edge cancer research.